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enzyme-linked immunosorbent assay

  • Improved Early Detection of Focal Brucellosis Complications with Anti-<em>Brucella</em> IgG
    Bacteriology
    Improved Early Detection of Focal Brucellosis Complications with Anti-Brucella IgG

    To evaluate the associations of inflammatory factors and serological test results with complicated brucellosis, we recruited 285 patients with a diagnosis of brucellosis between May 2016 and September 2019. The patients were subsequently classified into two groups according to the presence of complications. We collected demographic and clinical information and routine laboratory test results in addition to anti-Brucella IgG and...

    Nannan Xu, Xiaomeng Dong, Yongyuan Yao, Yanyan Guan, Fengzhe Chen, Feng Zheng, Gang Wang
  • Open Access
    A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays
    Immunoassays
    A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

    We set out to investigate the interference factors that led to false-positive novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM detection results using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions. GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (...

    Qiang Wang, Qin Du, Bin Guo, Daiyong Mu, Xiaolan Lu, Qiang Ma, Yangliu Guo, Li Fang, Bing Zhang, Guoyuan Zhang, Xiaolan Guo
  • Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to <em>Coxiella burnetii</em>
    Immunoassays
    Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

    Several commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of phase II IgG or IgM antibodies against Coxiella burnetii were compared. In addition, an indirect immunofluorescence test was used as a confirmation test. In all, 70 serum samples for IgG and 43 serum samples for IgM were tested. The ELISAs showed large differences in...

    Larissa Dangel, Daniela Koempf, Silke F. Fischer
  • Immunoassays
    Performance Assessment of a Trypanosoma cruzi Chimeric Antigen in Multiplex Liquid Microarray Assays
    Fred Luciano Neves Santos, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Amanda Leitolis, Sandra Crestani, Leonardo Foti, Wayner Vieira de Souza, Yara de Miranda Gomes, Marco Aurélio Krieger
  • Open Access
    Virology
    Designing HIV Testing Algorithms Based on 2015 WHO Guidelines Using Data from Six Sites in Sub-Saharan Africa
    Cara S. Kosack, Leslie Shanks, Greet Beelaert, Tumwesigye Benson, Aboubacar Savane, Anne Ng'ang'a, André Bita, Jean-Paul B. N. Zahinda, Katrien Fransen, Anne-Laure Page
  • Open Access
    Immunoassays
    Improved Serodiagnostic Performance for Lyme Disease by Use of Two Recombinant Proteins in Enzyme-Linked Immunosorbent Assay Compared to Standardized Two-Tier Testing
    Gary L. Bradshaw, R. Kelley Thueson, Todd J. Uriona
  • Free
    Virology
    Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing
    Arnaud G. L'Huillier, Anne Hamid-Allie, Erik Kristjanson, Louis Papageorgiou, Sam Hung, Chun Fai Wong, Derek R. Stein, Romy Olsha, Lee W. Goneau, Kristina Dimitrova, Mike Drebot, David Safronetz, Jonathan B. Gubbay
  • Free
    Virology
    Serologic Testing for Zika Virus: Comparison of Three Zika Virus IgM-Screening Enzyme-Linked Immunosorbent Assays and Initial Laboratory Experiences
    Dane Granger, Heather Hilgart, Lori Misner, Jaime Christensen, Sarah Bistodeau, Jennifer Palm, Anna K. Strain, Marja Konstantinovski, Dakai Liu, Anthony Tran, Elitza S. Theel
  • Free
    Virology
    Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile
    Yaniv Lustig, Hana Zelena, Giulietta Venturi, Marjan Van Esbroeck, Camilla Rothe, Cecilia Perret, Ravit Koren, Shiri Katz-Likvornik, Ella Mendelson, Eli Schwartz
  • Mycology
    Prospective Biomarker Screening for Diagnosis of Invasive Aspergillosis in High-Risk Pediatric Patients
    Juergen Loeffler, Julia Hafner, Carlo Mengoli, Clemens Wirth, Claus Peter Heussel, Claudia Löffler, P. Lewis White, Andrew J. Ullmann, Denise Michel, Verena Wiegering, Matthias Wölfl, Paul Gerhardt Schlegel, Hermann Einsele, Jan Springer, Matthias Eyrich

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