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Journal of Clinical Microbiology
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Escherichia coli

  • Additional Og-Typing PCR Techniques Targeting <span class="named-content genus-species" id="named-content-1">Escherichia coli</span>-Novel and <em>Shigella</em>-Unique O-Antigen Biosynthesis Gene Clusters
    Bacteriology
    Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and Shigella-Unique O-Antigen Biosynthesis Gene Clusters

    The O-serogrouping of pathogenic Escherichia coli is a standard method for subtyping strains for epidemiological studies and controls. O-serogroup diversification shows a strong association with the genetic diversity in some O-antigen biosynthesis gene clusters. Through genomic studies, in addition to the types of O-antigen biosynthesis gene clusters (Og-types) from...

    Atsushi Iguchi, Hironobu Nishii, Kazuko Seto, Jiro Mitobe, Kenichi Lee, Noriko Konishi, Hiromi Obata, Taisei Kikuchi, Sunao Iyoda
  • Optimizing a Screening Protocol for Potential Extended-Spectrum β-Lactamase <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> on MacConkey Agar for Use in a Global Surveillance Program
    Bacteriology
    Optimizing a Screening Protocol for Potential Extended-Spectrum β-Lactamase Escherichia coli on MacConkey Agar for Use in a Global Surveillance Program

    The increasing prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is worrisome. Coordinated efforts to better understand global prevalence and risk factors are needed. Developing lower- and middle-income countries need reliable, readily available, and cost-effective solutions for detecting ESBL...

    Megan E. Jacob, Shivaramu Keelara, Awa Aidara-Kane, Jorge R. Matheu Alvarez, Paula J. Fedorka-Cray
  • Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Serogroups and Major Virulence Genes
    Clinical Veterinary Microbiology
    Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing Escherichia coli Serogroups and Major Virulence Genes

    Escherichia coli serogroups O157, O26, O45, O103, O111, O121, and O145, when carrying major virulence genes, the Shiga toxin genes stx1 and stx2 and the intimin gene eae, are important foodborne pathogens. They are referred to as the “top 7” Shiga toxin-producing...

    Xuming Liu, Lance Noll, Xiaorong Shi, Elizabeth Porter, Yin Wang, Colin Stoy, Nanyan Lu, T. G. Nagaraja, Gary Anderson, Jianfa Bai
  • Carbapenem-Susceptible <span class="named-content genus-species" id="named-content-1">Klebsiella pneumoniae</span> and <span class="named-content genus-species" id="named-content-2">Escherichia coli</span> Isolates Carrying a Truncated KPC Carbapenemase: a Challenge for Rapid Molecular Diagnostics
    Letter to the Editor
    Carbapenem-Susceptible Klebsiella pneumoniae and Escherichia coli Isolates Carrying a Truncated KPC Carbapenemase: a Challenge for Rapid Molecular Diagnostics
    Hossein Salimnia, Jennifer Veltman, Pranatharthi H. Chandrasekar, Jason M. Pogue, Ryan Mynatt, Tanaz Salimnia, Steven H. Marshall, Andrea M. Hujer, Robert A. Bonomo
  • Effect of Clinically Meaningful Antibiotic Concentrations on Recovery of <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> and <span class="named-content genus-species" id="named-content-2">Klebsiella pneumoniae</span> Isolates from Anaerobic Blood Culture Bottles with and without Antibiotic Binding Resins
    Bacteriology
    Effect of Clinically Meaningful Antibiotic Concentrations on Recovery of Escherichia coli and Klebsiella pneumoniae Isolates from Anaerobic Blood Culture Bottles with and without Antibiotic Binding Resins

    Blood cultures are routinely collected in pairs of aerobic and anaerobic bottles. Artificial sterilization of Gram-negative bacteria in aerobic bottles containing clinically meaningful antibiotic concentrations has previously been observed. This study assessed recovery from anaerobic bottles with and without antibiotic binding resins.

    Iris H. Chen, David P. Nicolau, Joseph L. Kuti
  • Rapid Emergence, Subsidence, and Molecular Detection of <em>Escherichia coli</em> Sequence Type 1193-<em>fimH64</em>, a New Disseminated Multidrug-Resistant Commensal and Extraintestinal Pathogen
    Epidemiology
    Rapid Emergence, Subsidence, and Molecular Detection of Escherichia coli Sequence Type 1193-fimH64, a New Disseminated Multidrug-Resistant Commensal and Extraintestinal Pathogen

    Escherichia coli sequence type 1193 (ST1193) is an emerging multidrug-resistant pathogen. We performed longitudinal and cross-sectional surveillance for ST1193 among clinical and fecal E. coli isolates from Minneapolis Veterans Affairs Medical Center (VAMC) patients and their household members,...

    James R. Johnson, Brian D. Johnston, Stephen B. Porter, Connie Clabots, Tricia L. Bender, Paul Thuras, Darren J. Trott, Rowland Cobbold, Joanne Mollinger, Patricia Ferrieri, Sarah Drawz, Ritu Banerjee
  • Rapid Detection of Fosfomycin Resistance in <span class="named-content genus-species" id="named-content-1">Escherichia coli</span>
    Bacteriology
    Rapid Detection of Fosfomycin Resistance in Escherichia coli

    The rapid fosfomycin/Escherichia coli NP test was developed to detect fosfomycin resistance in E. coli isolates. The test is based on glucose metabolization and the detection of bacterial growth in the presence of fosfomycin at 40 µg/ml.

    Patrice Nordmann, Laurent Poirel, Linda Mueller
  • Open Access
    Evaluation of a Culture-Dependent Algorithm and a Molecular Algorithm for Identification of <span class="named-content genus-species" id="named-content-1">Shigella</span> spp., <span class="named-content genus-species" id="named-content-2">Escherichia coli</span>, and Enteroinvasive <span class="named-content genus-species" id="named-content-3">E. coli</span>
    Bacteriology
    Evaluation of a Culture-Dependent Algorithm and a Molecular Algorithm for Identification of Shigella spp., Escherichia coli, and Enteroinvasive E. coli

    Identification of Shigella spp., Escherichia coli, and enteroinvasive E. coli (EIEC) is challenging because of their close relatedness. Distinction is vital, as infections with...

    Maaike J. C. van den Beld, Richard F. de Boer, Frans A. G. Reubsaet, John W. A. Rossen, Kai Zhou, Sjoerd Kuiling, Alexander W. Friedrich, Mirjam A. M. D. Kooistra-Smid
  • Clinical Veterinary Microbiology
    Population Structure and Antimicrobial Resistance of Canine Uropathogenic Escherichia coli
    Tessa E. LeCuyer, Barbara A. Byrne, Joshua B. Daniels, Dubraska V. Diaz-Campos, G. Kenitra Hammac, Claire B. Miller, Thomas E. Besser, Margaret A. Davis
  • Bacteriology
    Escherichia coli H-Genotyping PCR: a Complete and Practical Platform for Molecular H Typing
    Masaya Banjo, Atsushi Iguchi, Kazuko Seto, Taisei Kikuchi, Tetsuya Harada, Flemming Scheutz, Sunao Iyoda
    and Pathogenic E. coli Working Group in Japan

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