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hepatitis B virus

  • A Highly Prevalent Polymorphism in the Core Region Impairs Quantification of Hepatitis B Virus (HBV) by the cobas TaqMan HBV Assay
    Virology
    A Highly Prevalent Polymorphism in the Core Region Impairs Quantification of Hepatitis B Virus (HBV) by the cobas TaqMan HBV Assay

    The high genetic variability of hepatitis B virus (HBV) can impair DNA quantification. Here, we investigate a major underquantification of HBV by the cobas TaqMan HBV assay (CTM; Roche). In France, between 2005 and 2017, HBV DNA was detected in 3,102 blood donations by use of the CTM (95% limit of detection [LOD95], 4.8 IU/ml). HBV strains were sequenced in the S region (LOD95, ∼30 IU/ml). Concordant (n = ...

    Laure Boizeau, Annabelle Servant-Delmas, Alexandra Ducancelle, Stéphane Chevaliez, Vincent Thibault, Syria Laperche, Pierre Cappy
  • Open Access
    Comparison of Serum Hepatitis B Virus RNA Levels and Quasispecies Evolution Patterns between Entecavir and Pegylated-Interferon Mono-treatment in Chronic Hepatitis B Patients
    Virology
    Comparison of Serum Hepatitis B Virus RNA Levels and Quasispecies Evolution Patterns between Entecavir and Pegylated-Interferon Mono-treatment in Chronic Hepatitis B Patients

    Hepatitis B virus (HBV) RNA may independently predict virological and serological response. This study aimed to compare dynamic changes in serum HBV RNA levels and HBV quasispecies evolution patterns between entecavir and pegylated-interferon mono-treatment in chronic hepatitis B patients and to determine the clinical significance during treatment. TaqMan real-time PCR was used for quantitative analysis. HBV RNA levels were...

    Xiao-qi Yu, Ming-jie Wang, De-min Yu, Pei-zhan Chen, Ming-yu Zhu, Wei Huang, Yue Han, Qi-ming Gong, Xin-xin Zhang
  • Diagnostic Value of Detection of Pregenomic RNA in Sera of Hepatitis B Virus-Infected Patients with Different Clinical Outcomes
    Virology
    Diagnostic Value of Detection of Pregenomic RNA in Sera of Hepatitis B Virus-Infected Patients with Different Clinical Outcomes

    Pregenomic RNA (pgRNA) is a direct transcription product of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), and it plays important roles in viral genome amplification and replication. This study was designed to investigate whether serum pgRNA is a strong alternative marker for reflecting HBV cccDNA levels and to analyze the correlation between serum pgRNA, serum HBV DNA, and hepatitis B surface antigen (HBsAg).

    ...
    Ni Lin, Aizhu Ye, Jinpiao Lin, Can Liu, Jinlan Huang, Ya Fu, Songhang Wu, Siyi Xu, Long Wang, Qishui Ou
  • Open Access
    Virology
    High Prevalence and Diversity of Hepatitis Viruses in Suspected Cases of Yellow Fever in the Democratic Republic of Congo
    Sheila Makiala-Mandanda, Frédéric Le Gal, Nadine Ngwaka-Matsung, Steve Ahuka-Mundeke, Richard Onanga, Berthold Bivigou-Mboumba, Elisabeth Pukuta-Simbu, Athenaïs Gerber, Jessica L. Abbate, Dieudonné Mwamba, Nicolas Berthet, Eric Maurice Leroy, Jean-Jacques Muyembe-Tamfum, Pierre Becquart
  • Virology
    The New Aptima HBV Quant Real-Time TMA Assay Accurately Quantifies Hepatitis B Virus DNA from Genotypes A to F
    Stéphane Chevaliez, Claude Dauvillier, Fabienne Dubernet, Jean-Dominique Poveda, Syria Laperche, Christophe Hézode, Jean-Michel Pawlotsky
  • Virology
    Sensitive Assay for Quantification of Hepatitis B Virus Mutants by Use of a Minor Groove Binder Probe and Peptide Nucleic Acids
    Shuhei Hige, Yoichi Yamamoto, Shigeru Yoshida, Tomoe Kobayashi, Hiromasa Horimoto, Keiko Yamamoto, Takuya Sho, Mitsuteru Natsuizaka, Mitsuru Nakanishi, Makoto Chuma, Masahiro Asaka
  • Virology
    Hepatitis B Virus (HBV) Subgenotypes C2 and B2 Differ in Lamivudine- and Adefovir-Resistance-Associated Mutational Patterns in HBV-Infected Chinese Patients
    Xiaodong Li, Lin Wang, Yanwei Zhong, Vincent Wai-Sun Wong, Zhihui Xu, Yan Liu, Qinghong Li, Shaojie Xin, Jingmin Zhao, Dongping Xu
  • Virology
    Robust Hepatitis B Virus Genotyping by Mass Spectrometry
    L. Ganova-Raeva, S. Ramachandran, C. Honisch, J. C. Forbi, X. Zhai, Y. Khudyakov
  • Virology
    Microarray for Hepatitis B Virus Genotyping and Detection of 994 Mutations along the Genome
    Marie Gauthier, Bertrand Bonnaud, Maud Arsac, Fabien Lavocat, Jérôme Maisetti, Alan Kay, François Simon, Fabien Zoulim, Guy Vernet
  • Epidemiology
    Unique Hepatitis B Virus Subgenotype in a Primitive Tribal Community in Eastern India
    Sumantra Ghosh, Priyanka Banerjee, Arindam RoyChoudhury, Sumanta Sarkar, Alip Ghosh, Amal Santra, Soma Banerjee, Kausik Das, Bhagirathi Dwibedi, Shantanu K. Kar, Vikas Gangadhar Rao, Jyothi T. Bhat, Neeru Singh, Abhijit Chowdhury, Simanti Datta

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