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immunoassay

  • Free
    Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens
    Immunoassays
    Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens

    Reliable serological tests are required to determine the prevalence of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to characterize immunity to the disease in order to address key knowledge gaps in the coronavirus disease 2019 (COVID-19) pandemic. Quantitative suspension array technology (qSAT) assays based on the xMAP Luminex platform overcome the limitations of rapid diagnostic tests and enzyme-...

    Carlota Dobaño, Marta Vidal, Rebeca Santano, Alfons Jiménez, Jordi Chi, Diana Barrios, Gemma Ruiz-Olalla, Natalia Rodrigo Melero, Carlo Carolis, Daniel Parras, Pau Serra, Paula Martínez de Aguirre, Francisco Carmona-Torre, Gabriel Reina, Pere Santamaria, Alfredo Mayor, Alberto L. García-Basteiro, Luis Izquierdo, Ruth Aguilar, Gemma Moncunill
  • Free
    Detection of SARS-CoV-2 IgG Targeting Nucleocapsid or Spike Protein by Four High-Throughput Immunoassays Authorized for Emergency Use
    Immunoassays
    Detection of SARS-CoV-2 IgG Targeting Nucleocapsid or Spike Protein by Four High-Throughput Immunoassays Authorized for Emergency Use

    A total of 1,200 serum samples that were tested for SARS-CoV-2 IgG antibody using the Abbott Architect immunoassay targeting the nucleocapsid protein were run in 3 SARS-CoV-2 IgG immunoassays targeting spike proteins (DiaSorin Liaison, Ortho Vitros, and Euroimmun). Consensus-positive and consensus-negative interpretations were defined as qualitative agreement in at least 3 of the 4 assays. Agreement of the 4 individual assays with a...

    Harry E. Prince, Tara S. Givens, Mary Lapé-Nixon, Nigel J. Clarke, Dale A. Schwab, Hollis J. Batterman, Robert S. Jones, William A. Meyer, Hema Kapoor, Charles M. Rowland, Farnoosh Haji-Sheikhi, Elizabeth M. Marlowe
  • Open Access
    Development and Validation of the Elecsys Anti-SARS-CoV-2 Immunoassay as a Highly Specific Tool for Determining Past Exposure to SARS-CoV-2
    Virology
    Development and Validation of the Elecsys Anti-SARS-CoV-2 Immunoassay as a Highly Specific Tool for Determining Past Exposure to SARS-CoV-2

    The Elecsys Anti-SARS-CoV-2 immunoassay (Roche Diagnostics) was developed to provide accurate, reliable detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutralization assay for the Elecsys Anti-SARS-CoV-2 immunoassay. Sensitivity and agreement between Elecsys Anti-SARS-CoV-2...

    Peter Muench, Simon Jochum, Verena Wenderoth, Beatus Ofenloch-Haehnle, Michael Hombach, Matthias Strobl, Henrik Sadlowski, Christopher Sachse, Giulia Torriani, Isabella Eckerle, Alexander Riedel
  • Development and Evaluation of a New Triplex Immunoassay That Detects Group A <em>Streptococcus</em> Antibodies for the Diagnosis of Rheumatic Fever
    Immunoassays
    Development and Evaluation of a New Triplex Immunoassay That Detects Group A Streptococcus Antibodies for the Diagnosis of Rheumatic Fever

    Streptococcal serology is a cornerstone in the diagnosis of acute rheumatic fever (ARF), a postinfectious sequela associated with group A Streptococcus infection. Current tests that measure anti-streptolysin O (ASO) and anti-DNaseB (ADB) titers require parallel processing, with their predictive value limited by the low rate of decay in antibody response. Accordingly, our objective was to develop and assess the diagnostic...

    Alana L. Whitcombe, Paulina Hanson-Manful, Susan Jack, Arlo Upton, Polly Atatoa Carr, Deborah A. Williamson, Michael G. Baker, Thomas Proft, Nicole J. Moreland
  • A Fully Automated Multiplex Assay for Diagnosis of Lyme Disease with High Specificity and Improved Early Sensitivity
    Immunoassays
    A Fully Automated Multiplex Assay for Diagnosis of Lyme Disease with High Specificity and Improved Early Sensitivity

    Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferi sensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi. The BioPlex 2200 Lyme Total assay exhibits an...

    Johnnie B. Hahm, John W. Breneman, Jing Liu, Svetlana Rabkina, Weiming Zheng, Shuxia Zhou, Roger P. Walker, Ravi Kaul
  • Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for <span class="named-content genus-species" id="named-content-1">Streptococcus pneumoniae</span>: a Non-Culture-Based Approach for Serotype-Specific Detection
    Immunoassays
    Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection

    The multiplexed immunoassay (MIA) is an automated, monoclonal antibody-based serotyping assay that uses culture lysates of Streptococcus pneumoniae. This study describes the development and validation of applying MIA directly to sputum samples for the serotype-specific detection of S. pneumoniae....

    Sun Jin Kim, Yoo Jung Jeong, Jong Hun Kim, Young Kyung Yoon, Jang Wook Sohn, Moon Hee Nahm, Min Ja Kim
  • Open Access
    Immunoassays
    Performance Evaluation of a Prototype Architect Antibody Assay for Babesia microti

    The tick-borne protozoan Babesia microti is responsible for more than 200 cases of transfusion-transmitted babesiosis (TTB) infection in the United States that have occurred over the last 30 years. Measures to mitigate the risk of TTB include nucleic acid testing (NAT) and B. microti antibody...

    Kevin Cheng, Kelly E. Coller, Christopher C. Marohnic, Zachary A. Pfeiffer, James R. Fino, Randee R. Elsing, Janet Bergsma, Marilee A. Marcinkus, Alak K. Kar, Orlando H. Gumbs, Kathy S. Otis, Jeffrey Fishpaugh, Phillip W. Schultz, Mark R. Pope, Alfredo R. Narvaez, Susan J. Wong, Susan Madison-Antenucci, Thomas P. Leary, George J. Dawson
  • Immunoassays
    Development and Evaluation of a Broad Bead-Based Multiplex Immunoassay To Measure IgG Seroreactivity against Human Polyomaviruses
    Sergio Kamminga, Els van der Meijden, Herman F. Wunderink, Antoine Touzé, Hans L. Zaaijer, Mariet C. W. Feltkamp
  • Immunoassays
    Evaluation of the Lumipulse G TP-N Chemiluminescent Immunoassay as a Syphilis Screening Test
    Daniel A. Ortiz, Michael J. Loeffelholz
  • Mycology
    Usefulness of Two Aspergillus PCR Assays and Aspergillus Galactomannan and β-d-Glucan Testing of Bronchoalveolar Lavage Fluid for Diagnosis of Chronic Pulmonary Aspergillosis
    Naohisa Urabe, Susumu Sakamoto, Go Sano, Junko Suzuki, Akira Hebisawa, Yasuhiko Nakamura, Kazuya Koyama, Yoshikazu Ishii, Kazuhiro Tateda, Sakae Homma

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