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lateral flow assay

  • Open Access
    Diagnostic Accuracy of the Biosynex CryptoPS Cryptococcal Antigen Semiquantitative Lateral Flow Assay in Patients with Advanced HIV Disease
    Mycology
    Diagnostic Accuracy of the Biosynex CryptoPS Cryptococcal Antigen Semiquantitative Lateral Flow Assay in Patients with Advanced HIV Disease

    High cryptococcal antigen (CrAg) titers in blood are associated with subclinical meningitis and mortality in CrAg-positive individuals with advanced HIV disease (AHD). We evaluated a novel semiquantitative lateral flow assay (LFA), CryptoPS, that may be able to identify individuals with high CrAg titers in a cohort of AHD patients undergoing CrAg screening. In a prospective cohort of patients with AHD (CD4 cell count, ≤200/μl) receiving...

    Mark W. Tenforde, Timothée Boyer-Chammard, Charles Muthoga, Leabaneng Tawe, Thandi Milton, Ikanyeng Rulaganyang, Kwana Lechiile, Ivy Rukasha, Tshepo B. Leeme, Nelesh P. Govender, Julia Ngidi, Madisa Mine, Síle F. Molloy, Thomas S. Harrison, Olivier Lortholary, Joseph N. Jarvis
  • Open Access
    Evaluation of a Novel Semiquantitative Cryptococcal Antigen Lateral Flow Assay in Patients with Advanced HIV Disease
    Mycology
    Evaluation of a Novel Semiquantitative Cryptococcal Antigen Lateral Flow Assay in Patients with Advanced HIV Disease

    Higher cryptococcal antigen (CrAg) titers are strongly associated with mortality risk in individuals with HIV-associated cryptococcal disease. Rapid tests to quantify CrAg levels may provide important prognostic information and enable treatment stratification. We performed a laboratory-based validation of the IMMY semiquantitative cryptococcal antigen (CrAgSQ) lateral flow assay (LFA) against the current gold standard CrAg tests. We...

    Joseph N. Jarvis, Mark W. Tenforde, Kwana Lechiile, Thandi Milton, Amber Boose, Tshepo B. Leeme, Leabaneng Tawe, Charles Muthoga, Ivy Rukasha, Fredah Mulenga, Ikanyeng Rulaganyang, Mooketsi Molefi, Síle F. Molloy, Julia Ngidi, Thomas S. Harrison, Nelesh P. Govender, Madisa Mine
  • Free
    Evaluation of the COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech
    Special Issue Immunoassays
    Evaluation of the COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech

    While the coronavirus disease 2019 (COVID-19) pandemic has peaked in many countries already, the current challenge is to assess population immunity on a large scale. Many serological tests are available and require urgent independent validation. Here, we report performance characteristics of Orient Gene Biotech COVID-19 IgG/IgM Rapid Test Cassette (OG) and compare it to Abbott SARS-CoV-2 IgG immunoassay (ASIA). Patients (n = ...

    Sarah Dellière, Maud Salmona, Marine Minier, Audrey Gabassi, Alexandre Alanio, Jérôme Le Goff, Constance Delaugerre, Marie-Laure Chaix
    and on behalf of Saint-Louis CORE (COvid REsearch) group
  • Evaluation of the Performance of the IMMY sona <em>Aspergillus</em> Galactomannan Lateral Flow Assay When Testing Serum To Aid in Diagnosis of Invasive Aspergillosis
    Mycology
    Evaluation of the Performance of the IMMY sona Aspergillus Galactomannan Lateral Flow Assay When Testing Serum To Aid in Diagnosis of Invasive Aspergillosis

    Management of invasive aspergillosis has been improved by biomarker assays, but limited accessibility and batch testing limit the impact. Lateral flow assays (LFA) are a simple method for use outside specialist centers, provided performance is acceptable. The objective of this study was to determine the performance of the recently released IMMY sona Aspergillus LFA when testing serum samples. The study took the form of a...

    P. Lewis White, Jessica S. Price, Raquel Posso, Morgan Cutlan-Vaughan, Lorna Vale, Matthijs Backx
  • Open Access
    Evaluation of LDBio <em>Aspergillus</em> ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis
    Immunoassays
    Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis

    Detecting Aspergillus-specific IgG is critical to diagnosing chronic pulmonary aspergillosis (CPA). Existing assays are often cost- and resource-intensive and not compatible with resource-constrained laboratory settings. LDBio Diagnostics has recently commercialized a lateral flow assay based on immunochromatographic technology (ICT) that detects Aspergillus antibodies (IgG and IgM) in less than 30 min, requiring...

    Elizabeth Stucky Hunter, Malcolm D. Richardson, David W. Denning
  • Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates
    Minireview
    Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates

    The rapid spread of multidrug-resistant Gram-negative organisms constitutes one of the greatest challenges to global health. While Gram-negative organisms have developed several mechanisms to avert the bactericidal effects of commonly prescribed antibiotic agents, the increasing prevalence of carbapenemase-producing organisms (CPO) is particularly concerning given the rapid spread of mobile genetic elements containing carbapenemase...

    Pranita D. Tamma, Patricia J. Simner
  • Immunoassays
    Low Cryptococcus Antigen Titers as Determined by Lateral Flow Assay Should Be Interpreted Cautiously in Patients without Prior Diagnosis of Cryptococcal Infection
    Marie Dubbels, Dane Granger, Elitza S. Theel
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