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Journal of Clinical Microbiology
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MALDI-TOF MS

  • Novel Strategy for Rapidly and Safely Distinguishing <span class="named-content genus-species" id="named-content-1">Bacillus anthracis</span> and <span class="named-content genus-species" id="named-content-2">Bacillus cereus</span> by Use of Peptide Mass Fingerprints Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
    Bacteriology
    Novel Strategy for Rapidly and Safely Distinguishing Bacillus anthracis and Bacillus cereus by Use of Peptide Mass Fingerprints Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

    The objective of this study was to construct a rapid, high-throughput, and biosafety-compatible screening method for Bacillus anthracis and Bacillus cereus based on matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS coupled to ClinProTools was...

    Jianchun Wei, Huijuan Zhang, Huifang Zhang, Enmin Zhang, Binghua Zhang, Fei Zhao, Di Xiao
  • Update on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identification of Filamentous Fungi
    Minireview
    Update on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identification of Filamentous Fungi

    Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In...

    Laura S. Wilkendorf, Edmée Bowles, Jochem B. Buil, Henrich A. L. van der Lee, Brunella Posteraro, Maurizio Sanguinetti, Paul E. Verweij
  • Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria
    Mycobacteriology and Aerobic Actinomycetes
    Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria

    Identification of mycobacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) requires not only a good protein extraction protocol but also an adequate cutoff score in order to provide reliable results. The aim of this study was to assess the cutoff scores proposed by the MALDI-TOF MS system for mycobacterial identification. A total of 693 clinical isolates from a liquid medium and 760...

    David Rodriguez-Temporal, Belén Rodríguez-Sánchez, Fernando Alcaide
  • Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper
    Mycology
    Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper

    MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB...

    Marie Gladys Robert, Charlotte Romero, Céline Dard, Cécile Garnaud, Odile Cognet, Thomas Girard, Tahinamandranto Rasamoelina, Muriel Cornet, Danièle Maubon
  • Performance of Five Commercial Identification Platforms for Identification of <span class="named-content genus-species" id="named-content-1">Staphylococcus delphini</span>
    Bacteriology
    Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini

    The Staphylococcus intermedius group (SIG) is a collection of coagulase-positive staphylococci consisting of four distinct species, namely, Staphylococcus cornubiensis, Staphylococcus delphini,...

    Matthew C. Canver, Tsigereda Tekle, Samantha T. Compton, Katrina Callan, Eileen M. Burd, Barbara L. Zimmer, David A. Bemis, Karen C. Carroll, Lars F. Westblade
  • Back to the Basics: Biochemical Testing for Pathogen Identification in the Era of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)
    Letter to the Editor
    Back to the Basics: Biochemical Testing for Pathogen Identification in the Era of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)
    Utsav Pandey, Samia N. Naccache, Jennifer Dien Bard
  • Open Access
    Species Distinction in the <span class="named-content genus-species" id="named-content-1">Trichophyton rubrum</span> Complex
    Mycology
    Species Distinction in the Trichophyton rubrum Complex

    The Trichophyton rubrum species complex comprises commonly encountered dermatophytic fungi with a worldwide distribution. The members of the complex usually have distinct phenotypes in culture and cause different clinical symptoms, despite high genome similarity. In order to better delimit the species within the complex, molecular, phenotypic, and physiological...

    Huilin Su, Ann Packeu, Sarah A. Ahmed, Abdullah M. S. Al-Hatmi, Oliver Blechert, Macit İlkit, Ferry Hagen, Yvonne Gräser, Weida Liu, Shuwen Deng, Marijke Hendrickx, Jinhua Xu, Min Zhu, Sybren de Hoog
  • Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Is a Superior Diagnostic Tool for the Identification and Differentiation of Mycoplasmas Isolated from Animals
    Clinical Veterinary Microbiology
    Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Is a Superior Diagnostic Tool for the Identification and Differentiation of Mycoplasmas Isolated from Animals

    In veterinary diagnostic laboratories, identification of mycoplasmas is achieved by demanding, cost-intensive, and time-consuming methods that rely on antigenic or genetic identification.

    Joachim Spergser, Claudia Hess, Igor Loncaric, Ana S. Ramírez
  • Free
    <span class="named-content genus-species" id="named-content-1">Candida auris</span> Clinical Isolates from South Korea: Identification, Antifungal Susceptibility, and Genotyping
    Mycology
    Candida auris Clinical Isolates from South Korea: Identification, Antifungal Susceptibility, and Genotyping

    Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea.

    Yong Jun Kwon, Jong Hee Shin, Seung A Byun, Min Ji Choi, Eun Jeong Won, Dain Lee, Seung Yeob Lee, Sejong Chun, Jun Hyung Lee, Hyun Jung Choi, Seung Jung Kee, Soo Hyun Kim, Myung Geun Shin
  • Answer to March 2019 Photo Quiz
    Photo Quiz
    Answer to March 2019 Photo Quiz
    Tsubasa West, Rhonda A. Warren, Siu-Kei Chow

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