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Journal of Clinical Microbiology
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mass spectrometry

  • Open Access
    A Phylogeny-Informed Proteomics Approach for Species Identification within the <span class="named-content genus-species" id="named-content-1">Burkholderia cepacia</span> Complex
    Bacteriology
    A Phylogeny-Informed Proteomics Approach for Species Identification within the Burkholderia cepacia Complex

    Ancestral genetic exchange between members of many important bacterial pathogen groups has resulted in phylogenetic relationships better described as networks than as bifurcating trees. In certain cases, these reticulated phylogenies have resulted in phenotypic and molecular overlap that challenges the construction of practical approaches for species identification in the clinical microbiology laboratory....

    Honghui Wang, Ousmane H. Cissé, Thomas Bolig, Steven K. Drake, Yong Chen, Jeffrey R. Strich, Jung-Ho Youn, Uchenna Okoro, Avi Z. Rosenberg, Junfeng Sun, John J. LiPuma, Anthony F. Suffredini, John P. Dekker
  • Discriminative Potential of the Vitek MS <em>In Vitro</em> Diagnostic Device Regarding <span class="named-content genus-species" id="named-content-1">Haemophilus influenzae</span> and <span class="named-content genus-species" id="named-content-2">Haemophilus haemolyticus</span>
    Letter to the Editor
    Discriminative Potential of the Vitek MS In Vitro Diagnostic Device Regarding Haemophilus influenzae and Haemophilus haemolyticus
    Sebastian Nürnberg, Heike Claus, Manuel Krone, Ulrich Vogel, Thiên-Trí Lâm
  • Open Access
    Detection of Colistin Resistance in <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> by Use of the MALDI Biotyper Sirius Mass Spectrometry System
    Bacteriology
    Detection of Colistin Resistance in Escherichia coli by Use of the MALDI Biotyper Sirius Mass Spectrometry System

    Polymyxin antibiotics are a last-line treatment for multidrug-resistant Gram-negative bacteria. However, the emergence of colistin resistance, including the spread of mobile mcr genes, necessitates the development of improved diagnostics for the detection of colistin-resistant organisms in hospital settings.

    R. Christopher D. Furniss, Laurent Dortet, William Bolland, Oliver Drews, Katrin Sparbier, Rémy A. Bonnin, Alain Filloux, Markus Kostrzewa, Despoina A. I. Mavridou, Gerald Larrouy-Maumus
  • Identification of the OXA-48 Carbapenemase Family by Use of Tryptic Peptides and Liquid Chromatography-Tandem Mass Spectrometry
    Bacteriology
    Identification of the OXA-48 Carbapenemase Family by Use of Tryptic Peptides and Liquid Chromatography-Tandem Mass Spectrometry

    Phenotypic detection of the OXA-48-type class D β-lactamases in Enterobacteriaceae is challenging. We describe a rapid (less than 90 min) assay for the identification of OXA-48 family carbapenemases in subcultured bacterial isolates based on a genoproteomic approach.

    Jeffrey R. Strich, Honghui Wang, Ousmane H. Cissé, Jung-Ho Youn, Steven K. Drake, Yong Chen, Avi Z. Rosenberg, Marjan Gucek, Patrick T. McGann, John P. Dekker, Anthony F. Suffredini
  • Open Access
    Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers
    Mycology
    Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers

    A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay.

    Marjorie Cornu, Boualem Sendid, Alexandre Mery, Nadine François, Malgorzata Mikulska, Valérie Letscher-Bru, Elena De Carolis, Lauro Damonti, Marie Titecat, Pierre-Yves Bochud, Alexandre Alanio, Maurizio Sanguinetti, Claudio Viscoli, Raoul Herbrecht, Yann Guerardel, Daniel Poulain
  • A Prospective Study of <em>Acinetobacter baumannii</em> Complex Isolates and Colistin Susceptibility Monitoring by Mass Spectrometry of Microbial Membrane Glycolipids
    Bacteriology
    A Prospective Study of Acinetobacter baumannii Complex Isolates and Colistin Susceptibility Monitoring by Mass Spectrometry of Microbial Membrane Glycolipids

    Acinetobacter baumannii is a prevalent nosocomial pathogen with a high incidence of multidrug resistance. Treatment of infections due to this organism with colistin, a last-resort antibiotic of the polymyxin class, can result in the emergence of colistin-resistant strains.

    Lisa M. Leung, Christi L. McElheny, Francesca M. Gardner, Courtney E. Chandler, Sarah L. Bowler, Roberta T. Mettus, Caressa N. Spychala, Erin L. Fowler, Belita N. A. Opene, Robert A. Myers, David R. Goodlett, Yohei Doi, Robert K. Ernst
  • Free
    The Brief Case
    Closing the Brief Case: An Unusual Cause of Infective Endocarditis after a Urological Procedure
    Kévin Diallo, Janina Ferrand, François Goehringer, Christine Selton-Suty, Thierry Folliguet, Corentine Alauzet, Alain Lozniewski
  • Free
    The Brief Case
    The Brief Case: An Unusual Cause of Infective Endocarditis after a Urological Procedure
    Kévin Diallo, Janina Ferrand, François Goehringer, Christine Selton-Suty, Thierry Folliguet, Corentine Alauzet, Alain Lozniewski
  • Mycobacteriology and Aerobic Actinomycetes
    Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species
    Barbara A. Body, Melodie A. Beard, E. Susan Slechta, Kimberly E. Hanson, Adam P. Barker, N. Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A. Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J. Wallace, S. Turner, L. Curtis, Susan Butler-Wu, Jenna Rychert
  • Bacteriology
    Improved Discrimination of Bacillus anthracis from Closely Related Species in the Bacillus cereus Sensu Lato Group Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
    Viktoria I. Pauker, Bryan R. Thoma, Gregor Grass, Pauline Bleichert, Matthias Hanczaruk, Lothar Zöller, Sabine Zange

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