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molecular tests

  • Open Access
    Storage of Sputum in Cetylpyridinium Chloride, OMNIgene.SPUTUM, and Ethanol Is Compatible with Molecular Tuberculosis Diagnostic Testing
    Mycobacteriology and Aerobic Actinomycetes
    Storage of Sputum in Cetylpyridinium Chloride, OMNIgene.SPUTUM, and Ethanol Is Compatible with Molecular Tuberculosis Diagnostic Testing

    We compared cetylpyridinium chloride (CPC), ethanol (ETOH), and OMNIgene.SPUTUM (OMNI) for 28-day storage of sputum at ambient temperature before molecular tuberculosis diagnostics. Three sputum samples were collected from each of 133 smear-positive tuberculosis (TB) patients (399 sputum samples).

    C. N’Dira Sanoussi, Bouke C. de Jong, Dissou Affolabi, Conor J. Meehan, Mathieu Odoun, Leen Rigouts
  • Heat Inactivation Renders Sputum Safe and Preserves <em>Mycobacterium tuberculosis</em> RNA for Downstream Molecular Tests
    Mycobacteriology and Aerobic Actinomycetes
    Heat Inactivation Renders Sputum Safe and Preserves Mycobacterium tuberculosis RNA for Downstream Molecular Tests

    The World Health Organization End Tuberculosis (TB) strategy has called for the development of—and increased access to—effective tools for diagnosis and treatment of TB disease. Mycobacterium tuberculosis , the causative agent of TB, is categorized as a highly infectious agent.

    Wilber Sabiiti, Khalide Azam, Ezembro Esmeraldo, Nilesh Bhatt, Andrea Rachow, Stephen H. Gillespie
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