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mutation

  • Open Access
    Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR
    Letter to the Editor
    Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR
    Manu Vanaerschot, Sabrina A. Mann, James T. Webber, Jack Kamm, Sidney M. Bell, John Bell, Si Noon Hong, Minh Phuong Nguyen, Lienna Y. Chan, Karan D. Bhatt, Michelle Tan, Angela M. Detweiler, Alex Espinosa, Wesley Wu, Joshua Batson, David Dynerman, Debra A. Wadford, Andreas S. Puschnik, Norma Neff, Vida Ahyong, Steve Miller, Patrick Ayscue, Cristina M. Tato, Simon Paul, Amy L. Kistler, Joseph L. DeRisi, Emily D. Crawford
  • Diagnostic Accuracy of Pan-Degenerate Amplification and Adaptation Assay for HIV-1 Drug Resistance Mutation Analysis in Low- and Middle-Income Countries
    Virology
    Diagnostic Accuracy of Pan-Degenerate Amplification and Adaptation Assay for HIV-1 Drug Resistance Mutation Analysis in Low- and Middle-Income Countries

    HIV drug resistance (HIVDR) is a barrier to sustained virologic suppression in low- and middle-income countries (LMICs). Point mutation assays targeting priority drug resistance mutations (DRMs) are being evaluated to improve access to HIVDR testing. In a cross-sectional study (June 2018 to September 2019), we evaluated the diagnostic accuracy of a simple and rapid HIVDR assay (the pan-degenerate amplification and adaptation [PANDAA]...

    Vinie Kouamou, Justen Manasa, David Katzenstein, Alan M. McGregor, Chiratidzo E. Ndhlovu, Tariro Makadzange
  • Identification of an Unusual 16S rRNA Mutation in <span class="named-content genus-species" id="named-content-1">Neisseria gonorrhoeae</span>
    Letter to the Editor
    Identification of an Unusual 16S rRNA Mutation in Neisseria gonorrhoeae
    Christine J. D. Guglielmino, Sally Appleton, Renu Vohra, Amy V. Jennison
  • GenoType NTM-DR Performance Evaluation for Identification of <span class="named-content genus-species" id="named-content-1">Mycobacterium avium</span> Complex and <span class="named-content genus-species" id="named-content-2">Mycobacterium abscessus</span> and Determination of Clarithromycin and Amikacin Resistance
    Mycobacteriology and Aerobic Actinomycetes
    GenoType NTM-DR Performance Evaluation for Identification of Mycobacterium avium Complex and Mycobacterium abscessus and Determination of Clarithromycin and Amikacin Resistance

    We evaluated the GenoType NTM-DR (NTM-DR) line probe assay for identifying Mycobacterium avium complex (MAC) species and Mycobacterium abscessus subspecies and for determining clarithromycin and amikacin resistance. Thirty-eight reference strains and 145 clinical isolates (58 MAC and 87...

    Hee Jae Huh, Su-Young Kim, Hyang Jin Shim, Dae Hun Kim, In Young Yoo, On-Kyun Kang, Chang-Seok Ki, So Youn Shin, Byung Woo Jhun, Sung Jae Shin, Charles L. Daley, Won-Jung Koh, Nam Yong Lee
  • <span class="named-content genus-species" id="named-content-1">Helicobacter pylori</span> Mutations Detected by Next-Generation Sequencing in Formalin-Fixed, Paraffin-Embedded Gastric Biopsy Specimens Are Associated with Treatment Failure
    Bacteriology
    Helicobacter pylori Mutations Detected by Next-Generation Sequencing in Formalin-Fixed, Paraffin-Embedded Gastric Biopsy Specimens Are Associated with Treatment Failure

    Helicobacter pylori antibiotic resistance is widespread and increasing worldwide. Routine detection of H. pylori mutations that invoke antimicrobial resistance may be a useful approach to guide antimicrobial therapy and possibly avert treatment failure.

    Behtash G. Nezami, Mehul Jani, David Alouani, Daniel D. Rhoads, Navid Sadri
  • Bacteriology
    Mycoplasma genitalium Macrolide and Fluoroquinolone Resistance Detection and Clinical Implications in a Selected Cohort in New Zealand
    Trevor Anderson, Edward Coughlan, Anja Werno
  • Virology
    Differentiated Levels of Ganciclovir Resistance Conferred by Mutations at Codons 591 to 603 of the Cytomegalovirus UL97 Kinase Gene
    Sunwen Chou, Ronald J. Ercolani, Adam L. Vanarsdall
  • Open Access
    Mycobacteriology and Aerobic Actinomycetes
    Use of GeneXpert Remnants for Drug Resistance Profiling and Molecular Epidemiology of Tuberculosis in Libreville, Gabon
    Amel Kévin Alame-Emane, Catherine Pierre-Audigier, Oriane Cordelia Aboumegone-Biyogo, Amandine Nzoghe-Mveang, Véronique Cadet-Daniel, Christophe Sola, Joël Fleury Djoba-Siawaya, Brigitte Gicquel, Howard E. Takiff
  • Open Access
    Virology
    Detection of Emerging Vaccine-Related Polioviruses by Deep Sequencing
    Malaya K. Sahoo, Marisa Holubar, ChunHong Huang, Alisha Mohamed-Hadley, Yuanyuan Liu, Jesse J. Waggoner, Stephanie B. Troy, Lourdes Garcia-Garcia, Leticia Ferreyra-Reyes, Yvonne Maldonado, Benjamin A. Pinsky
  • Mycobacteriology and Aerobic Actinomycetes
    Increased Tuberculosis Patient Mortality Associated with Mycobacterium tuberculosis Mutations Conferring Resistance to Second-Line Antituberculous Drugs
    Sophia B. Georghiou, Marva Seifert, Donald G. Catanzaro, Richard S. Garfein, Timothy C. Rodwell

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