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mycobacteria

  • Open Access
    DNA Thermo-Protection Facilitates Whole-Genome Sequencing of Mycobacteria Direct from Clinical Samples
    Mycobacteriology and Aerobic Actinomycetes
    DNA Thermo-Protection Facilitates Whole-Genome Sequencing of Mycobacteria Direct from Clinical Samples

    Mycobacterium tuberculosis is the leading cause of death from bacterial infection. Improved rapid diagnosis and antimicrobial resistance determination, such as by whole-genome sequencing, are required. Our aim was to develop a simple, low-cost method of preparing DNA for sequencing direct from M....

    Sophie George, Yifei Xu, Gillian Rodger, Marcus Morgan, Nicholas D. Sanderson, Sarah J. Hoosdally, Samantha Thulborn, Esther Robinson, Priti Rathod, A. Sarah Walker, Timothy E. A. Peto, Derrick W. Crook, Kate E. Dingle
  • Antimycobacterial Susceptibility Testing of Nontuberculous Mycobacteria
    Minireview
    Antimycobacterial Susceptibility Testing of Nontuberculous Mycobacteria

    Recommendations for first-line and second-line drug testing and organism group, specific methodologies, and reporting recommendations have been addressed by the Clinical and Laboratory Standards Institute (CLSI) and are important in the selection of appropriate antimicrobial treatment regimens for nontuberculous mycobacteria (NTM) disease.

    Barbara A. Brown-Elliott, Gail L. Woods
  • An Update on Mycobacterial Taxonomy, 2016–2017
    Minireview
    An Update on Mycobacterial Taxonomy, 2016–2017
    Nicole Parrish
  • Implementation of Semiautomated Antimicrobial Susceptibility Interpretation Hardware for Nontuberculous Mycobacteria May Overestimate Susceptibility
    Mycobacteriology and Aerobic Actinomycetes
    Implementation of Semiautomated Antimicrobial Susceptibility Interpretation Hardware for Nontuberculous Mycobacteria May Overestimate Susceptibility

    Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimicrobial susceptibility testing (AST) to determine antibiotic minimum inhibitory concentrations (MICs).

    ...
    Mariëlle Rockland, Mike Marvin Ruth, Nicole Aalders, Lian Pennings, Wouter Hoefsloot, Melanie Wattenberg, Jakko van Ingen
  • Mycobacteriology and Aerobic Actinomycetes
    Performance of Vitek MS v3.0 for Identification of Mycobacterium Species from Patient Samples by Use of Automated Liquid Medium Systems

    The accuracy and robustness of the Vitek MS v3.0 matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) system was evaluated by identifying mycobacteria from automated liquid-medium systems using patient samples. This is the first report to demonstrate that proteins within the liquid medium, its supplements, and decontamination reagents for nonsterile patient samples do not generate...

    Eric Miller, Christopher Cantrell, Melodie Beard, Andrew Derylak, N. Esther Babady, Tracy McMillen, Edwin Miranda, Barbara Body, Yi-Wei Tang, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Elena Iakhiaeva, Richard J. Wallace, Barbara A. Brown-Elliott, Erik Moreno, Heather Totty, Parampal Deol
  • Mycobacteriology and Aerobic Actinomycetes
    Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species
    Barbara A. Body, Melodie A. Beard, E. Susan Slechta, Kimberly E. Hanson, Adam P. Barker, N. Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A. Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J. Wallace, S. Turner, L. Curtis, Susan Butler-Wu, Jenna Rychert
  • Open Access
    Mycobacteriology and Aerobic Actinomycetes
    Evaluation of Whole-Genome Sequencing for Mycobacterial Species Identification and Drug Susceptibility Testing in a Clinical Setting: a Large-Scale Prospective Assessment of Performance against Line Probe Assays and Phenotyping
    T. Phuong Quan, Zharain Bawa, Dona Foster, Tim Walker, Carlos del Ojo Elias, Priti Rathod, MMM Informatics Group, Zamin Iqbal, Phelim Bradley, Janet Mowbray, A. Sarah Walker, Derrick W. Crook, David H. Wyllie, Timothy E. A. Peto, E. Grace Smith
  • Mycobacteriology and Aerobic Actinomycetes
    Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria
    Pieter-Jan Ceyssens, Karine Soetaert, Markus Timke, An Van den Bossche, Katrin Sparbier, Koen De Cremer, Markus Kostrzewa, Marijke Hendrickx, Vanessa Mathys
  • Minireview
    Mycobacterial Taxonomy
    Betty A. Forbes
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