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Journal of Clinical Microbiology
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nontuberculous mycobacteria

  • Open Access
    GenSeizer: a Multiplex PCR-Based Targeted Gene Sequencing Platform for Rapid and Accurate Identification of Major <em>Mycobacterium</em> Species
    Mycobacteriology and Aerobic Actinomycetes
    GenSeizer: a Multiplex PCR-Based Targeted Gene Sequencing Platform for Rapid and Accurate Identification of Major Mycobacterium Species

    Mycobacterium tuberculosis and nontuberculous mycobacterium (NTM) infections often exhibit similar clinical symptoms. Timely and effective treatment relies on the rapid and accurate identification of species and resistance genotypes.

    Bing Li, Liyun Xu, Qi Guo, Jianhui Chen, Yanan Zhang, Wenpan Huang, Zhemin Zhang, Lizhong Han, Xiaogang Xu, Haiqing Chu
  • Antimicrobial Susceptibility and Phylogenetic Relations in a German Cohort Infected with <span class="named-content genus-species" id="named-content-1">Mycobacterium abscessus</span>
    Mycobacteriology and Aerobic Actinomycetes
    Antimicrobial Susceptibility and Phylogenetic Relations in a German Cohort Infected with Mycobacterium abscessus

    Mycobacterium abscessus is a highly antibiotic-resistant opportunistic pathogen causing clinically challenging infections in patients with preexisting lung diseases or under immunosuppression. Hence, reliable antibiotic susceptibility data are required for effective treatment. Aims of this study were to investigate (i) the congruence of genotypic and phenotypic...

    Nils Wetzstein, Thomas A. Kohl, Tilman G. Schultze, Sönke Andres, Carla Bellinghausen, Christian Hügel, Volkhard A. J. Kempf, Annette Lehn, Michael Hogardt, Hubert Serve, Maria J. G. T. Vehreschild, Timo Wolf, Stefan Niemann, Florian P. Maurer, Thomas A. Wichelhaus
  • Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria
    Mycobacteriology and Aerobic Actinomycetes
    Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria

    Identification of mycobacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) requires not only a good protein extraction protocol but also an adequate cutoff score in order to provide reliable results. The aim of this study was to assess the cutoff scores proposed by the MALDI-TOF MS system for mycobacterial identification. A total of 693 clinical isolates from a liquid medium and 760...

    David Rodriguez-Temporal, Belén Rodríguez-Sánchez, Fernando Alcaide
  • Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major <em>Mycobacterium</em> Species
    Mycobacteriology and Aerobic Actinomycetes
    Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species

    Mycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for...

    Guohui Xiao, Xing He, Su Zhang, Yaya Liu, Zhihang Liang, Houming Liu, Juanjuan Zhang, Min Ou, Shuhao Cai, Wenjie Lai, Tianyu Zhang, Lili Ren, Guoliang Zhang
  • Open Access
    Recovery of Nontuberculous Mycobacteria and Nocardiae Causing Skin/Soft Tissue Infections by Use of the Copan ESwab Collection and Transport System
    Letter to the Editor
    Recovery of Nontuberculous Mycobacteria and Nocardiae Causing Skin/Soft Tissue Infections by Use of the Copan ESwab Collection and Transport System
    B. Gandhi, G. Woods, T. Mazzulli
  • Antimycobacterial Susceptibility Testing of Nontuberculous Mycobacteria
    Minireview
    Antimycobacterial Susceptibility Testing of Nontuberculous Mycobacteria

    Recommendations for first-line and second-line drug testing and organism group, specific methodologies, and reporting recommendations have been addressed by the Clinical and Laboratory Standards Institute (CLSI) and are important in the selection of appropriate antimicrobial treatment regimens for nontuberculous mycobacteria (NTM) disease.

    Barbara A. Brown-Elliott, Gail L. Woods
  • GenoType NTM-DR Performance Evaluation for Identification of <span class="named-content genus-species" id="named-content-1">Mycobacterium avium</span> Complex and <span class="named-content genus-species" id="named-content-2">Mycobacterium abscessus</span> and Determination of Clarithromycin and Amikacin Resistance
    Mycobacteriology and Aerobic Actinomycetes
    GenoType NTM-DR Performance Evaluation for Identification of Mycobacterium avium Complex and Mycobacterium abscessus and Determination of Clarithromycin and Amikacin Resistance

    We evaluated the GenoType NTM-DR (NTM-DR) line probe assay for identifying Mycobacterium avium complex (MAC) species and Mycobacterium abscessus subspecies and for determining clarithromycin and amikacin resistance. Thirty-eight reference strains and 145 clinical isolates (58 MAC and 87...

    Hee Jae Huh, Su-Young Kim, Hyang Jin Shim, Dae Hun Kim, In Young Yoo, On-Kyun Kang, Chang-Seok Ki, So Youn Shin, Byung Woo Jhun, Sung Jae Shin, Charles L. Daley, Won-Jung Koh, Nam Yong Lee
  • Performance of RGM Medium for Isolation of Nontuberculous Mycobacteria from Respiratory Specimens from Non-Cystic Fibrosis Patients
    Mycobacteriology and Aerobic Actinomycetes
    Performance of RGM Medium for Isolation of Nontuberculous Mycobacteria from Respiratory Specimens from Non-Cystic Fibrosis Patients

    A new selective medium for rapidly growing mycobacteria (RGM medium) was evaluated on respiratory specimens from non-cystic fibrosis patients and compared to the mycobacterial growth indicator tube (MGIT) system and Middlebrook 7H11 agar for the isolation of all nontuberculous mycobacteria (NTM). A total of 203 mucolyzed respiratory specimens collected from 163 patients were inoculated on RGM medium and incubated at both 30°C (RGM30)...

    Suwatchareeporn Rotcheewaphan, Oluwadamilola E. Odusanya, Christina M. Henderson, Dominic Stephenson, Kenneth N. Olivier, John D. Perry, Adrian M. Zelazny
  • Rapid Identification of Clinically Relevant <em>Mycobacterium</em> Species by Multicolor Melting Curve Analysis
    Mycobacteriology and Aerobic Actinomycetes
    Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis

    The sustained increase in the incidence of nontuberculous mycobacterial (NTM) infection and the difficulty in distinguishing these infections from tuberculosis constitute an urgent need for NTM species-level identification. The MeltPro Myco assay is the first diagnostic system that identifies 19 clinically relevant mycobacteria in a single reaction based on multicolor melting curve analysis run on a real-time PCR platform.

    ...
    Ye Xu, Bin Liang, Chen Du, Xueshan Tian, Xingshan Cai, Yanjie Hou, Hui Li, Rongrong Zheng, Junlian Li, Yuqin Liu, Kaili Wang, Muhammad Ammar Athar, Yaoju Tan, Qingge Li
  • Mycobacteriology and Aerobic Actinomycetes
    Performance of a Highly Sensitive Mycobacterium tuberculosis Complex Real-Time PCR Assay for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence Setting: a Prospective Intervention Study
    Víctor Vinuesa, Rafael Borrás, María Luisa Briones, María Ángeles Clari, Vicenta Cresencio, Estela Giménez, Carmen Muñoz, Rosa Oltra, Emilio Servera, Talia Scheelje, Carlos Tornero, David Navarro

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