nontuberculous mycobacteria
Mycobacteriology and Aerobic ActinomycetesEvaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous MycobacteriaIdentification of mycobacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) requires not only a good protein extraction protocol but also an adequate cutoff score in order to provide reliable results. The aim of this study was to assess the cutoff scores proposed by the MALDI-TOF MS system for mycobacterial identification. A total of 693 clinical isolates from a liquid medium and 760...
Mycobacteriology and Aerobic ActinomycetesCas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium SpeciesMycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for...
MinireviewAntimycobacterial Susceptibility Testing of Nontuberculous MycobacteriaRecommendations for first-line and second-line drug testing and organism group, specific methodologies, and reporting recommendations have been addressed by the Clinical and Laboratory Standards Institute (CLSI) and are important in the selection of appropriate antimicrobial treatment regimens for nontuberculous mycobacteria (NTM) disease.
Mycobacteriology and Aerobic ActinomycetesGenoType NTM-DR Performance Evaluation for Identification of Mycobacterium avium Complex and Mycobacterium abscessus and Determination of Clarithromycin and Amikacin ResistanceWe evaluated the GenoType NTM-DR (NTM-DR) line probe assay for identifying Mycobacterium avium complex (MAC) species and Mycobacterium abscessus subspecies and for determining clarithromycin and amikacin resistance. Thirty-eight reference strains and 145 clinical isolates (58 MAC and 87...
Mycobacteriology and Aerobic ActinomycetesPerformance of RGM Medium for Isolation of Nontuberculous Mycobacteria from Respiratory Specimens from Non-Cystic Fibrosis PatientsA new selective medium for rapidly growing mycobacteria (RGM medium) was evaluated on respiratory specimens from non-cystic fibrosis patients and compared to the mycobacterial growth indicator tube (MGIT) system and Middlebrook 7H11 agar for the isolation of all nontuberculous mycobacteria (NTM). A total of 203 mucolyzed respiratory specimens collected from 163 patients were inoculated on RGM medium and incubated at both 30°C (RGM30)...
Mycobacteriology and Aerobic ActinomycetesRapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis...The sustained increase in the incidence of nontuberculous mycobacterial (NTM) infection and the difficulty in distinguishing these infections from tuberculosis constitute an urgent need for NTM species-level identification. The MeltPro Myco assay is the first diagnostic system that identifies 19 clinically relevant mycobacteria in a single reaction based on multicolor melting curve analysis run on a real-time PCR platform.
- Mycobacteriology and Aerobic ActinomycetesDevelopment of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species
