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real-time RT-PCR

  • Biennial Upsurge and Molecular Epidemiology of Enterovirus D68 Infection in New York, USA, 2014 to 2018
    Virology
    Biennial Upsurge and Molecular Epidemiology of Enterovirus D68 Infection in New York, USA, 2014 to 2018

    Enterovirus D68 (EV-D68) infection has been associated with outbreaks of severe respiratory illness and increased cases of nonpolio acute flaccid myelitis. The patterns of EV-D68 circulation and molecular epidemiology are not fully understood. In this study, nasopharyngeal (NP) specimens collected from patients in the Lower Hudson Valley, New York, from 2014 to 2018 were examined for rhinovirus/enterovirus (RhV/EV) by the FilmArray...

    Victoria L. Gilrane, Jian Zhuge, Weihua Huang, Sheila M. Nolan, Abhay Dhand, Changhong Yin, Christian Salib, Faariah Shakil, Helen Engel, John T. Fallon, Guiqing Wang
  • Open Access
    Comparison of Four Molecular <em>In Vitro</em> Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens
    Special Issue Virology
    Comparison of Four Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel human coronavirus that causes coronavirus disease 2019 (COVID-19), was first discovered in December 2019 as the cause of an outbreak of pneumonia in the city of Wuhan, Hubei province, China. The clinical presentation of COVID-19 is fairly nonspecific, and symptoms overlap those of other seasonal respiratory infections concurrently circulating in the population....

    Wei Zhen, Ryhana Manji, Elizabeth Smith, Gregory J. Berry
  • Simultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay
    Virology
    Simultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay

    Measles is one of the most contagious viral respiratory infections and was declared to be eliminated from Canada in 1998; however, measles cases and outbreaks still occur every year through reintroduction from other parts of the world. Laboratory confirmation of measles virus (MV) RNA by real-time PCR provides a definitive diagnosis, and molecular analysis to determine the genotype is the only way to distinguish between wild-type and...

    Kanti Pabbaraju, Kara Gill, Anita A. Wong, Graham A. Tipples, Joanne Hiebert, Alberto Severini, Kevin Fonseca, Raymond Tellier
  • Monitoring Viral Genetic Variation as a Tool To Improve Molecular Diagnostics for Mumps Virus
    Virology
    Monitoring Viral Genetic Variation as a Tool To Improve Molecular Diagnostics for Mumps Virus

    Here, we report how the analysis of viral genetic variation using next-generation sequencing (NGS) can be used as a tool to improve mumps virus diagnostics. Analysis of NGS data from recently circulating mumps virus isolates allowed optimization of the current mumps virus real-time reverse transcription-PCR (RT-PCR) by primer and probe modifications due to nucleotide variations.

    Meik Dilcher, Kevin Barratt, Jennifer Douglas, Andrew Strathdee, Trevor Anderson, Anja Werno
  • Virology
    Development of a Real-Time Reverse Transcription-PCR Assay for Global Differentiation of Yellow Fever Virus Vaccine-Related Adverse Events from Natural Infections
    Holly R. Hughes, Brandy J. Russell, Eric C. Mossel, John Kayiwa, Julius Lutwama, Amy J. Lambert
  • Virology
    Monitoring Shedding of Five Genotypes of RotaTeq Vaccine Viruses by Genotype-Specific Real-Time Reverse Transcription-PCR Assays
    Yuki Higashimoto, Masaru Ihira, Yu Miyazaki, Ayumi Kuboshiki, Sayaka Yoshinaga, Hiroyuki Hiramatsu, Ryota Suzuki, Masafumi Miyata, Hiroki Miura, Satoshi Komoto, Jun Yukitake, Koki Taniguchi, Yoshiki Kawamura, Tetsushi Yoshikawa
  • Virology
    Performance Characteristics of a New Consensus Commercial Kit for Hepatitis D Virus RNA Viral Load Quantification
    Frédéric Le Gal, Samira Dziri, Athenaïs Gerber, Chakib Alloui, Zahia Ben Abdesselam, Dominique Roulot, Ségolène Brichler, Emmanuel Gordien
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