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Journal of Clinical Microbiology
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respiratory viruses

  • Free
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19
    Virology
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19

    Broad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of...

    Ahmed Babiker, Heath L. Bradley, Victoria D. Stittleburg, Jessica M. Ingersoll, Autum Key, Colleen S. Kraft, Jesse J. Waggoner, Anne Piantadosi
  • Open Access
    Multicenter Evaluation of the BioFire FilmArray Pneumonia/Pneumonia Plus Panel for Detection and Quantification of Agents of Lower Respiratory Tract Infection
    Bacteriology
    Multicenter Evaluation of the BioFire FilmArray Pneumonia/Pneumonia Plus Panel for Detection and Quantification of Agents of Lower Respiratory Tract Infection

    The ability to provide timely identification of the causative agents of lower respiratory tract infections can promote better patient outcomes and support antimicrobial stewardship efforts. Current diagnostic testing options include culture, molecular testing, and antigen detection. These methods may require collection of various specimens, involve extensive sample treatment, and can suffer from low sensitivity and long turnaround times...

    Caitlin N. Murphy, Randal Fowler, Joan Miquel Balada-Llasat, Amanda Carroll, Hanna Stone, Oluseun Akerele, Blake Buchan, Sam Windham, Amanda Hopp, Shira Ronen, Ryan F. Relich, Rebecca Buckner, Del A. Warren, Romney Humphries, Shelly Campeau, Holly Huse, Suki Chandrasekaran, Amy Leber, Kathy Everhart, Amanda Harrington, Christina Kwong, Andrew Bonwit, Jennifer Dien Bard, Samia Naccache, Cynthia Zimmerman, Barbara Jones, Cory Rindlisbacher, Maggie Buccambuso, Angela Clark, Margarita Rogatcheva, Corrin Graue, Kevin M. Bourzac
  • Optimal Timing of Repeat Multiplex Molecular Testing for Respiratory Viruses
    Virology
    Optimal Timing of Repeat Multiplex Molecular Testing for Respiratory Viruses

    Determining whether and when multiplex nucleic acid amplification tests (NAATs) for respiratory viruses should be repeated is difficult. We analyzed 5 years of results for a multiplex NAAT targeting 14 respiratory viruses, to determine how often repeat tests were ordered and the time period in which results were likely to change. Results for NAATs performed on nasopharyngeal specimens and repeated within 90 days after initial testing...

    Yamini Mandelia, Gary W. Procop, Sandra S. Richter, Sarah Worley, Wei Liu, Frank Esper
  • Open Access
    Cost-Effective Respiratory Virus Testing
    Minireview
    Cost-Effective Respiratory Virus Testing

    The timely and accurate diagnosis of respiratory virus infections has the potential to optimize downstream (posttesting) use of limited health care resources, including antibiotics, antivirals, ancillary testing, and inpatient and emergency department beds.

    B. A. Pinsky, R. T. Hayden
  • Free
    Impact of Rapid Molecular Diagnostic Testing of Respiratory Viruses on Outcomes of Adults Hospitalized with Respiratory Illness: a Multicenter Quasi-experimental Study
    Virology
    Impact of Rapid Molecular Diagnostic Testing of Respiratory Viruses on Outcomes of Adults Hospitalized with Respiratory Illness: a Multicenter Quasi-experimental Study

    A standard multiplex PCR offers comprehensive testing for respiratory viruses. However, it has traditionally been performed in a referral laboratory with a lengthy turnaround time, which can reduce patient flow through the hospital.

    Nasir Wabe, Ling Li, Robert Lindeman, Ruth Yimsung, Maria R. Dahm, Susan McLennan, Kate Clezy, Johanna I. Westbrook, Andrew Georgiou
  • PCR Detection of Respiratory Pathogens in Asymptomatic and Symptomatic Adults
    Epidemiology
    PCR Detection of Respiratory Pathogens in Asymptomatic and Symptomatic Adults

    The frequency of viral respiratory pathogens in asymptomatic subjects is poorly defined. The aim of this study was to explore the prevalence of respiratory pathogens in the upper airways of asymptomatic adults, compared with a reference population of symptomatic patients sampled in the same centers during the same period.

    Nicklas Sundell, Lars-Magnus Andersson, Robin Brittain-Long, Pär-Daniel Sundvall, Åsa Alsiö, Magnus Lindh, Lars Gustavsson, Johan Westin
  • Diagnostic Performance of Multiplex Nucleic Acid Testing of Bronchoalveolar Lavage and Bronchial Wash Specimens for Respiratory Viral Pathogens
    Epidemiology
    Diagnostic Performance of Multiplex Nucleic Acid Testing of Bronchoalveolar Lavage and Bronchial Wash Specimens for Respiratory Viral Pathogens

    There is limited knowledge on the yield of performing multiplex nucleic acid testing (NAT) on multiple lower respiratory tract specimens from a single patient with a single instance of infection. We evaluated the performance characteristics of multiplex NAT assays performed concurrently on bronchoalveolar lavage (BAL) and bronchial wash (BW) specimens to detect respiratory pathogens.

    Satish Munigala, Carey-Ann D. Burnham, Neil W. Anderson, Stephen Y. Liang, Steven J. Lawrence, David K. Warren
  • Minireview
    Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Use of Clinical Laboratory Improvement Amendments of 1988 (CLIA)-Waived Point-of-Care Assays: a Paradigm Shift to Molecular Tests
    Marwan M. Azar, Marie L. Landry
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