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virology

  • Free
    Quantitative Measurement of Anti-SARS-CoV-2 Antibodies: Analytical and Clinical Evaluation
    Immunoassays
    Quantitative Measurement of Anti-SARS-CoV-2 Antibodies: Analytical and Clinical Evaluation

    The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection.

    Victoria Higgins, Anselmo Fabros, Vathany Kulasingam
  • Taxonomic Changes for Human and Animal Viruses, 2018 to 2020
    Minireview
    Taxonomic Changes for Human and Animal Viruses, 2018 to 2020

    The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recent advances in virus taxonomy structure by the International Committee on Taxonomy of Viruses inform this update.

    ...
    Michael J. Loeffelholz, Bradley W. Fenwick
  • Free
    Test Agreement between Roche Cobas 6800 and Cepheid GeneXpert Xpress SARS-CoV-2 Assays at High Cycle Threshold Ranges
    Special Issue Letter to the Editor
    Test Agreement between Roche Cobas 6800 and Cepheid GeneXpert Xpress SARS-CoV-2 Assays at High Cycle Threshold Ranges
    Kari Broder, Ahmed Babiker, Charles Myers, Terri White, Heather Jones, John Cardella, Eileen M. Burd, Charles E. Hill, Colleen S. Kraft
  • Open Access
    Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19
    Special Issue Letter to the Editor
    Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19
    Daniel D. Rhoads, Sree S. Cherian, Katharine Roman, Lisa M. Stempak, Christine L. Schmotzer, Navid Sadri
  • Open Access
    Identification of Infectious Agents in High-Throughput Sequencing Data Sets Is Easily Achievable Using Free, Cloud-Based Bioinformatics Platforms
    Letter to the Editor
    Identification of Infectious Agents in High-Throughput Sequencing Data Sets Is Easily Achievable Using Free, Cloud-Based Bioinformatics Platforms
    Joseph G. Chappell, Timothy Byaruhanga, Theocharis Tsoleridis, Jonathan K. Ball, C. Patrick McClure
  • Open Access
    Metagenomic Next-Generation Sequencing for Identification and Quantitation of Transplant-Related DNA Viruses
    Virology
    Metagenomic Next-Generation Sequencing for Identification and Quantitation of Transplant-Related DNA Viruses

    Infections with DNA viruses are frequent causes of morbidity and mortality in transplant recipients.

    Meredith L. Carpenter, Susanna K. Tan, Thomas Watson, Rowena Bacher, Vaishnavi Nagesh, Alain Watts, Gordon Bentley, Jenna Weber, ChunHong Huang, Malaya K. Sahoo, Armin Hinterwirth, Thuy Doan, Theodore Carter, Queeny Dong, Stéphane Gourguechon, Eric Harness, Sean Kermes, Srihari Radhakrishnan, Gongbo Wang, Alejandro Quiroz-Zárate, Jesus Ching, Benjamin A. Pinsky
  • Taxonomic Changes for Human and Animal Viruses, 2016 to 2018
    Minireview
    Taxonomic Changes for Human and Animal Viruses, 2016 to 2018

    The classification of viruses provides the structure necessary to appreciate their biological diversity. Herein, we provide an update to our previous review of changes in viral taxonomy, covering changes between 2016 and 2018.

    Michael J. Loeffelholz, Bradley W. Fenwick
  • PCR Detection of Respiratory Pathogens in Asymptomatic and Symptomatic Adults
    Epidemiology
    PCR Detection of Respiratory Pathogens in Asymptomatic and Symptomatic Adults

    The frequency of viral respiratory pathogens in asymptomatic subjects is poorly defined. The aim of this study was to explore the prevalence of respiratory pathogens in the upper airways of asymptomatic adults, compared with a reference population of symptomatic patients sampled in the same centers during the same period.

    Nicklas Sundell, Lars-Magnus Andersson, Robin Brittain-Long, Pär-Daniel Sundvall, Åsa Alsiö, Magnus Lindh, Lars Gustavsson, Johan Westin
  • Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture
    Virology
    Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

    Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic testing that allows simultaneous detection of a broad range of viruses, detailed taxonomic assignment, and detection of mutations associated with antiviral drug resistance. To enhance sensitivity for virus detection, we previously developed ViroCap, a targeted sequence capture panel designed to enrich nucleic acid from a comprehensive set of eukaryotic...

    Kristine M. Wylie, Todd N. Wylie, Richard Buller, Brandi Herter, Maria T. Cannella, Gregory A. Storch
  • Minireview
    Towards a Universal Molecular Microbiological Test
    Richard J. N. Allcock, Amy V. Jennison, David Warrilow

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