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Journal of Clinical Microbiology
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Xpert

  • Open Access
    Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV
    Mycobacteriology and Aerobic Actinomycetes
    Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV

    A nonsputum triage test to rule out tuberculosis (TB) disease is a WHO high-priority diagnostic, and a combinatory score based on a 3-gene host signature has shown promise in discriminating TB from other illnesses. We evaluated the accuracy of an early-prototype cartridge assay (“Xpert MTB Host Response” or Xpert-MTB-HR-Prototype) of this 3-gene signature on biobanked blood samples from people living with HIV (PLHIV) against a...

    Erik Södersten, Stefano Ongarello, Anna Mantsoki, Romain Wyss, David H. Persing, Sara Banderby, Linda Strömqvist Meuzelaar, Jacqueline Prieto, Devasena Gnanashanmugam, Purvesh Khatri, Samuel G. Schumacher, Claudia M. Denkinger
  • Free
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test
    Special Issue Virology
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test

    Nucleic acid amplification tests (NAATs) are the primary means of identifying acute infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Accurate and fast test results may permit more efficient use of protective and isolation resources and allow rapid therapeutic interventions. We evaluated the analytical and clinical performance characteristics of the Xpert Xpress SARS-CoV-2 (Xpert) test, a rapid,...

    Michael J. Loeffelholz, David Alland, Susan M. Butler-Wu, Utsav Pandey, Carlo Frederico Perno, Alice Nava, Karen C. Carroll, Heba Mostafa, Emma Davies, Ashley McEwan, Jennifer L. Rakeman, Randal C. Fowler, Jean-Michel Pawlotsky, Slim Fourati, Sukalyani Banik, Padmapriya P. Banada, Shobha Swaminathan, Soumitesh Chakravorty, Robert W. Kwiatkowski, Victor C. Chu, JoAnn Kop, Rajiv Gaur, Mandy L. Y. Sin, Duy Nguyen, Simranjit Singh, Na Zhang, David H. Persing
  • Free
    Saliva as an Alternate Specimen Source for Detection of SARS-CoV-2 in Symptomatic Patients Using Cepheid Xpert Xpress SARS-CoV-2
    Special Issue Letter to the Editor
    Saliva as an Alternate Specimen Source for Detection of SARS-CoV-2 in Symptomatic Patients Using Cepheid Xpert Xpress SARS-CoV-2
    Clare McCormick-Baw, Kristi Morgan, Donna Gaffney, Yareli Cazares, Karen Jaworski, Adrienne Byrd, Kyle Molberg, Dominick Cavuoti
  • Simple Processing of Formalin-Fixed Paraffin-Embedded Tissue for Accurate Testing with the Xpert MTB/RIF Assay
    Letter to the Editor
    Simple Processing of Formalin-Fixed Paraffin-Embedded Tissue for Accurate Testing with the Xpert MTB/RIF Assay
    Indre Budvytiene, Niaz Banaei
  • Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda
    Bacteriology
    Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda

    Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. We aimed to assess the effects of OMNIgene Sputum (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacterial growth indicator tube (MGIT) testing over periods of 15 and 8 days, respectively. Sputum samples were collected from newly diagnosed smear-positive patients...

    Elisa Ardizzoni, Patrick Orikiriza, Charles Ssuuna, Dan Nyehangane, Mourad Gumsboga, Ivan Mugisha Taremwa, Esther Turyashemererwa, Juliet Mwanga-Amumpaire, Céline Langendorf, Maryline Bonnet
  • Open Access
    Performance Evaluation of the Luminex Aries <span class="named-content genus-species" id="named-content-1">C. difficile</span> Assay in Comparison to Two Other Molecular Assays within a Multihospital Health Care Center
    Bacteriology
    Performance Evaluation of the Luminex Aries C. difficile Assay in Comparison to Two Other Molecular Assays within a Multihospital Health Care Center

    Clostridioides difficile infection (CDI) remain a serious issue in the United States. Fast and accurate diagnosis of CDI is paramount to achieve immediate infection control initiation, triaging, and isolation, as well as appropriate antibiotic treatment. However, both, over- and underdiagnosis can lead to adverse patient outcomes, such as unnecessary administration of...

    Stefan Juretschko, Ryhana Manji, Reeti Khare, Shubhagata Das, Sherry Dunbar
  • Open Access
    Storage of Sputum in Cetylpyridinium Chloride, OMNIgene.SPUTUM, and Ethanol Is Compatible with Molecular Tuberculosis Diagnostic Testing
    Mycobacteriology and Aerobic Actinomycetes
    Storage of Sputum in Cetylpyridinium Chloride, OMNIgene.SPUTUM, and Ethanol Is Compatible with Molecular Tuberculosis Diagnostic Testing

    We compared cetylpyridinium chloride (CPC), ethanol (ETOH), and OMNIgene.SPUTUM (OMNI) for 28-day storage of sputum at ambient temperature before molecular tuberculosis diagnostics. Three sputum samples were collected from each of 133 smear-positive tuberculosis (TB) patients (399 sputum samples).

    C. N’Dira Sanoussi, Bouke C. de Jong, Dissou Affolabi, Conor J. Meehan, Mathieu Odoun, Leen Rigouts
  • Virology
    Comparison of the Xpert Flu/RSV XC and Xpress Flu/RSV Assays

    Molecular diagnostics for influenza and respiratory syncytial virus (RSV) have become commonplace, and various tests and systems have been cleared by the FDA for use in the United States. We performed a retrospective study to compare the Cepheid Xpress Flu/RSV assay with the Xpert Flu/RSV XC assay, using laboratory-developed tests (LDTs) as the reference method.

    Elena B. Popowitch, Melissa B. Miller
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