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Journal of Clinical Microbiology
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Latest Articles

  • Open Access
    Evaluation of the Roche cobas MTB and MTB-RIF/INH Assays in Samples from Germany and Sierra Leone
    Mycobacteriology and Aerobic Actinomycetes
    Evaluation of the Roche cobas MTB and MTB-RIF/INH Assays in Samples from Germany and Sierra Leone

    The cobas MTB and MTB-RIF/INH assays allow for detection of Mycobacterium tuberculosis complex (MTBC) nucleic acid and rifampicin (RIF) and isoniazid (INH) resistance-associated mutations in an automated, high-throughput workflow. In this study, we evaluated the performance of these assays, employing samples from settings of low and high tuberculosis (TB) burdens....

    Darshaalini Nadarajan, Doris Hillemann, Rashidatu Kamara, Lynda Foray, Ousman S. Conteh, Matthias Merker, Stefan Niemann, Jasmine Lau, Merlin Njoya, Katharina Kranzer, Akos Somoskovi, Florian P. Maurer
  • Open Access
    Evaluating Specimen Quality and Results from a Community-Wide, Home-Based Respiratory Surveillance Study
    Epidemiology
    Evaluating Specimen Quality and Results from a Community-Wide, Home-Based Respiratory Surveillance Study

    While influenza and other respiratory pathogens cause significant morbidity and mortality, the community-based burden of these infections remains incompletely understood. The development of novel methods to detect respiratory infections is essential for mitigating epidemics and developing pandemic-preparedness infrastructure.

    Ashley E. Kim, Elisabeth Brandstetter, Naomi Wilcox, Jessica Heimonen, Chelsey Graham, Peter D. Han, Lea M. Starita, Denise J. McCulloch, Amanda M. Casto, Deborah A. Nickerson, Margaret M. Van de Loo, Jennifer Mooney, Misja Ilcisin, Kairsten A. Fay, Jover Lee, Thomas R. Sibley, Victoria Lyon, Rachel E. Geyer, Matthew Thompson, Barry R. Lutz, Mark J. Rieder, Trevor Bedford, Michael Boeckh, Janet A. Englund, Helen Y. Chu
    and on behalf of the Seattle Flu Study Investigators
  • Free
    Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays
    Immunoassays
    Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays

    In this study, we comprehensively analyzed multispecific antibody kinetics of different immunoglobulins in hospitalized patients with acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Three hundred fifty-four blood samples longitudinally obtained from 81 IgG-seroconverting progressed coronavirus disease 2019 (CoVID-19) patients were quantified for spike 1 (S1), S2, and nucleocapsid protein (NCP)-specific IgM...

    Georg Semmler, Marianna Theresia Traugott, Marianne Graninger, Wolfgang Hoepler, Tamara Seitz, Hasan Kelani, Mario Karolyi, Erich Pawelka, Sara Aragón de La Cruz, Elisabeth Puchhammer-Stöckl, Stephan Walter Aberle, Karin Stiasny, Alexander Zoufaly, Judith Helene Aberle, Lukas Weseslindtner
  • Free
    Performance of Saliva, Oropharyngeal Swabs, and Nasal Swabs for SARS-CoV-2 Molecular Detection: a Systematic Review and Meta-analysis
    Minireview
    Performance of Saliva, Oropharyngeal Swabs, and Nasal Swabs for SARS-CoV-2 Molecular Detection: a Systematic Review and Meta-analysis

    Nasopharyngeal (NP) swabs are considered the highest-yield sample for diagnostic testing for respiratory viruses, including SARS-CoV-2. The need to increase capacity for SARS-CoV-2 testing in a variety of settings, combined with shortages of sample collection supplies, have motivated a search for alternative sample types with high sensitivity.

    Rose A. Lee, Joshua C. Herigon, Andrea Benedetti, Nira R. Pollock, Claudia M. Denkinger
  • Free
    Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing
    Virology
    Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing

    The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here, we report a simple and efficient workflow for whole-genome sequencing utilizing one-step reverse transcription-PCR (RT-PCR) amplification on a microfluidic platform, followed by MiSeq amplicon sequencing.

    Tao Li, Hye Kyung Chung, Papa K. Pireku, Brett F. Beitzel, Mark A. Sanborn, Cynthia Y. Tang, Richard D. Hammer, Detlef Ritter, Xiu-Feng Wan, Irina Maljkovic Berry, Jun Hang
  • Genomic Surveillance and Improved Molecular Typing of <span class="named-content genus-species" id="named-content-1">Bordetella pertussis</span> Using wgMLST
    Bacteriology
    Genomic Surveillance and Improved Molecular Typing of Bordetella pertussis Using wgMLST

    Multilocus sequence typing (MLST) provides allele-based characterization of bacterial pathogens in a standardized framework. However, classical MLST schemes for Bordetella pertussis, the causative agent of whooping cough, seldom reveal diversity among the small number of gene targets and thereby fail to delineate population structure.

    Michael R. Weigand, Yanhui Peng, Hannes Pouseele, Dane Kania, Katherine E. Bowden, Margaret M. Williams, M. Lucia Tondella
  • Evaluation of the Accelerate Pheno System for Rapid Identification and Antimicrobial Susceptibility Testing of Positive Blood Culture Bottles Inoculated with Primary Sterile Specimens from Patients with Suspected Severe Infections
    Bacteriology
    Evaluation of the Accelerate Pheno System for Rapid Identification and Antimicrobial Susceptibility Testing of Positive Blood Culture Bottles Inoculated with Primary Sterile Specimens from Patients with Suspected Severe Infections

    The Accelerate Pheno system is approved for rapid identification and phenotypic antimicrobial susceptibility testing (AST) of microorganisms grown from positive blood cultures inoculated with blood from septic patients. We evaluated the performance of the system for identification and AST from positive blood culture bottles inoculated with primary sterile nonblood specimens from patients with suspected severe infections.

    ...
    V. Chapot, L. Effenberg, J. Dohmen-Ruetten, J. Buer, J. Kehrmann
  • Free
    Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories
    Virology
    Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories

    Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to support clinical diagnosis and epidemiological investigations. Recently, assays for large-scale detection of total antibodies (Ab), immunoglobulin G (IgG), and IgM against SARS-CoV-2 antigens have been developed, but there are limited data on the diagnostic accuracy of these assays.

    Lene H. Harritshøj, Mikkel Gybel-Brask, Shoaib Afzal, Pia R. Kamstrup, Charlotte S. Jørgensen, Marianne Kragh Thomsen, Linda Hilsted, Lennart Friis-Hansen, Pal B. Szecsi, Lise Pedersen, Lene Nielsen, Cecilie B. Hansen, Peter Garred, Trine-Line Korsholm, Susan Mikkelsen, Kirstine O. Nielsen, Bjarne K. Møller, Anne T. Hansen, Kasper K. Iversen, Pernille B. Nielsen, Rasmus B. Hasselbalch, Kamille Fogh, Jakob B. Norsk, Jonas Henrik Kristensen, Kristian Schønning, Nikolai S. Kirkby, Alex C. Y. Nielsen, Lone H. Landsy, Mette Loftager, Dorte K. Holm, Anna C. Nilsson, Susanne G. Sækmose, Birgitte Grum-Schwensen, Bitten Aagaard, Thøger G. Jensen, Dorte M. Nielsen, Henrik Ullum, Ram B. Dessau
  • Open Access
    Antibody Responses to Two Recombinant Treponemal Antigens (rp17 and TmpA) before and after Azithromycin Treatment for Yaws in Ghana and Papua New Guinea
    Epidemiology
    Antibody Responses to Two Recombinant Treponemal Antigens (rp17 and TmpA) before and after Azithromycin Treatment for Yaws in Ghana and Papua New Guinea

    WHO and its partners aim to interrupt yaws transmission in countries of endemicity and to certify others as being yaws-free. Transmission can be assessed using rapid plasma reagin (RPR) tests, reflecting current or recent infection, but RPR is operationally impractical.

    Nishanth Parameswaran, Oriol Mitjà, Christian Bottomley, Cynthia Kwakye, Wendy Houinei, Allan Pillay, Damien Danavall, Kai-Hua Chi, Ronald C. Ballard, Anthony W. Solomon, Cheng Y. Chen, Sibauk V. Bieb, Yaw Adu-Sarkodie, David C. W. Mabey, Kingsley Asiedu, Michael Marks, Diana L. Martin
  • Free
    Immunologic Testing for SARS-CoV-2 Infection from the Antigen Perspective
    Minireview
    Immunologic Testing for SARS-CoV-2 Infection from the Antigen Perspective

    Coronavirus disease 2019 (COVID-19), caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally as a severe pandemic. SARS-CoV-2 infection stimulates antigen-specific antibody responses.

    Dandan Li, Jinming Li

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Journal of Clinical Microbiology® (JCM) publishes the most current research related to the laboratory diagnosis of human and animal infections and the role of the laboratory in both the management of infectious diseases and the elucidation of the epidemiology of infections.

Editor in Chief: Dr. Alexander J. McAdam

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