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Journal of Clinical Microbiology
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Latest Articles

  • Free
    Combined Self-Collected Anterior Nasal and Oropharyngeal Specimens versus Provider-Collected Nasopharyngeal Swabs for the Detection of SARS-CoV-2
    Letter to the Editor
    Combined Self-Collected Anterior Nasal and Oropharyngeal Specimens versus Provider-Collected Nasopharyngeal Swabs for the Detection of SARS-CoV-2
    S. M. Shakir, A. P. Barker, D. R. Hillyard, N. Gilmore, J. W. Barrett, R. R. Orlandi, K. E. Hanson
  • Evaluation of Surrogate Tests for the Presence of <em>mecA</em>-Mediated Methicillin Resistance in <span class="named-content genus-species" id="named-content-1">Staphylococcus capitis</span>, <span class="named-content genus-species" id="named-content-2">Staphylococcus haemolyticus</span>, <span class="named-content genus-species" id="named-content-3">Staphylococcus hominis</span>, and <span class="named-content genus-species" id="named-content-4">Staphylococcus warneri</span>
    Bacteriology
    Evaluation of Surrogate Tests for the Presence of mecA-Mediated Methicillin Resistance in Staphylococcus capitis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus warneri

    Testing of staphylococci other than Staphylococcus aureus (SOSA) for mecA-mediated resistance is challenging. Isolates of Staphylococcus capitis, Staphylococcus haemolyticus,...

    Romney M. Humphries, Paul Magnano, Carey-Ann D. Burnham, Jennifer Dien Bard, Tanis C. Dingle, Katrina Callan, Lars F. Westblade
    and the Coagulase Negative Staphylococcus Ad Hoc Working Group
  • A Clinical Epidemiology and Molecular Attribution Evaluation of Adenoviruses in Pediatric Acute Gastroenteritis: a Case-Control Study
    Virology
    A Clinical Epidemiology and Molecular Attribution Evaluation of Adenoviruses in Pediatric Acute Gastroenteritis: a Case-Control Study

    The objective of this study was to characterize the etiological role of human adenovirus (HAdV) serotypes in pediatric gastroenteritis. Using a case-control design, we compared the frequencies of HAdV serotypes between children with ≥3 episodes of vomiting or diarrhea within 24 h and <7 days of symptoms (i.e., cases) and those with no infectious symptoms (i.e., controls). Stool samples and/or rectal swabs underwent molecular...

    Kanti Pabbaraju, Raymond Tellier, Xiao-Li Pang, Jianling Xie, Bonita E. Lee, Linda Chui, Ran Zhuo, Otto G. Vanderkooi, Samina Ali, Phillip I. Tarr, Anna Funk, Stephen B. Freedman
    and on behalf of the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE)
  • Free
    Sensitive Recovery of Complete SARS-CoV-2 Genomes from Clinical Samples by Use of Swift Biosciences’ SARS-CoV-2 Multiplex Amplicon Sequencing Panel
    Letter to the Editor
    Sensitive Recovery of Complete SARS-CoV-2 Genomes from Clinical Samples by Use of Swift Biosciences’ SARS-CoV-2 Multiplex Amplicon Sequencing Panel
    Amin Addetia, Michelle J. Lin, Vikas Peddu, Pavitra Roychoudhury, Keith R. Jerome, Alexander L. Greninger
  • Free
    COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva
    Immunoassays
    COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that detection of SARS-CoV-2 salivary antibodies could serve as a noninvasive alternative to serological testing for monitoring...

    Nora Pisanic, Pranay R. Randad, Kate Kruczynski, Yukari C. Manabe, David L. Thomas, Andrew Pekosz, Sabra L. Klein, Michael J. Betenbaugh, William A. Clarke, Oliver Laeyendecker, Patrizio P. Caturegli, H. Benjamin Larman, Barbara Detrick, Jessica K. Fairley, Amy C. Sherman, Nadine Rouphael, Srilatha Edupuganti, Douglas A. Granger, Steve W. Granger, Matthew H. Collins, Christopher D. Heaney
  • Global Epidemiology and Evolutionary History of <span class="named-content genus-species" id="named-content-1">Staphylococcus aureus</span> ST45
    Epidemiology
    Global Epidemiology and Evolutionary History of Staphylococcus aureus ST45

    Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome sequencing to analyze a large collection of 451...

    N. Effelsberg, M. Stegger, L. Peitzmann, O. Altinok, G. W. Coombs, B. Pichon, A. Kearns, P. R. Randad, C. D. Heaney, S. Bletz, F. Schaumburg, A. Mellmann
  • C-Reactive Protein Testing for Active Tuberculosis among Inpatients without HIV in Uganda: a Diagnostic Accuracy Study
    Epidemiology
    C-Reactive Protein Testing for Active Tuberculosis among Inpatients without HIV in Uganda: a Diagnostic Accuracy Study

    The objective of this prospective cross-sectional study, conducted at a national referral hospital in Kampala, Uganda, was to determine diagnostic performance of serum C-reactive protein (CRP) as a triage test for tuberculosis (TB) among HIV-seronegative inpatients. We calculated the sensitivity, specificity, positive and negative likelihood ratios, and positive and negative predictive values to determine the diagnostic performance of a...

    Amanda J. Meyer, Emmanuel Ochom, Patricia Turimumahoro, Patrick Byanyima, Ingvar Sanyu, Rejani Lalitha, Sylvia Kaswabuli, Alfred Andama, Nicholas D. Walter, Achilles Katamba, Adithya Cattamanchi, William Worodria, Laurence Huang, Christina Yoon, J. Lucian Davis
  • Open Access
    Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications
    Bacteriology
    Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications

    As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup B meningococcal disease (IMD) since their licensure in 2013 and 2014. These vaccines have different components and...

    Charlene M. C. Rodrigues, Keith A. Jolley, Andrew Smith, J. Claire Cameron, Ian M. Feavers, Martin C. J. Maiden
  • Free
    Application, Verification, and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization
    Commentary
    Application, Verification, and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization

    Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning...

    Elitza S. Theel, Marc Roger Couturier, Laura Filkins, Elizabeth Palavecino, Stephanie Mitchell, Sheldon Campbell, Michael Pentella, Susan Butler-Wu, Kurt Jerke, Vaishali Dharmarha, Peggy McNult, Audrey N. Schuetz
    and on behalf of the American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices
  • Free
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19
    Virology
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19

    Broad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of...

    Ahmed Babiker, Heath L. Bradley, Victoria D. Stittleburg, Jessica M. Ingersoll, Autum Key, Colleen S. Kraft, Jesse J. Waggoner, Anne Piantadosi

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Journal of Clinical Microbiology® (JCM) publishes the most current research related to the laboratory diagnosis of human and animal infections and the role of the laboratory in both the management of infectious diseases and the elucidation of the epidemiology of infections.

Editor in Chief: Dr. Alexander J. McAdam

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